A Proteomic Atlas of Senescence-Associated Secretomes for Aging Biomarker Development
Authors: Nathan Basisty 1 , Abhijit Kale 1 , Okhee Jeon 1 , Chisaka Kuehnemann 1 , Therese Payne 1 , Chirag Rao 1 , Anja Holtz 1 , Samah Shah 1 , Luigi Ferrucci 3 , Judith Campisi 1, 2 , Birgit Schilling ** 1
1 The Buck Institute for Research on Aging, Novato, California 94947, USA.
2 Lawence Berkeley Laboratory, University of California, Berkeley, California 94720,USA.
3 National Institute on Aging, Bethesda, Maryland 20892, USA.
** Correspondence: email@example.com
SUMMARY: The senescence-associated secretory phenotype (SASP) has recently emerged as both a driver of, and promising therapeutic target for, multiple age-related conditions, ranging from neurodegeneration to cancer. The complexity of the SASP,
typically monitored by a few dozen secreted proteins, has been greatly underappreciated, and a small set of factors cannot explain the diverse phenotypes it
produces in vivo. Here, we present ‘SASP Atlas’, a comprehensive proteomic database of soluble and exosome SASP factors originating from multiple senescence inducers
and cell types. Each profile consists of hundreds of largely distinct proteins, but also includes a subset of proteins elevated in all SASPs. Based on our analyses, we
propose several candidate biomarkers of cellular senescence, including GDF15, STC1 and SERPINs. This resource will facilitate identification of proteins that drive specific
senescence-associated phenotypes and catalog potential senescence biomarkers to assess the burden, originating stimulus and tissue of senescent cells in vivo.